Immunohistochemical investigation of cell cycle and apoptosis regulators (Survivin, β-Catenin, P53, Caspase 3) in canine appendicular osteosarcoma

Table 4 Primary antibodies used in immunohistochemical analysis

MOLECULE	TYPE OF Ab	COMPANY	WORKING DILUTION	POSITIVE CONTROL
β-catenin	Rabbit PAb	Santa Cruz Biotecnology	1:250	Normal canine skin [80]
Survivin	Rabbit PAb	NOVUS Biologicals	0.7 μg/ml	Canine cutaneous SCC [79]
p53	Rabbit PAb	NovoCastra	1:400	Canine nail bed SCC (Figure 5)
Procaspase 3	Rat MAb	NovoCastra	1:85	Normal canine tonsil [81]
Active Caspase 3	Mouse MAb	Oncogene	1:200	Canine breast carcinoma [81]

Clonality, company, working dilutions and positive controls used for each antibody. PAb: polyclonal antibody; MAb: monoclonal antibody. SCC: squamous cell carcinoma.

ISSN: 1746-6148