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Table 3 Immunohistochemical results 2

From: Immunohistochemical investigation of cell cycle and apoptosis regulators (Survivin, β-Catenin, P53, Caspase 3) in canine appendicular osteosarcoma

Grade

β-catenin

p53

 

N

M

C

 

I

-

-

-

-

0

2 (50%)

2 (50%)

-

2 (50%)

>0 - <10%

2 (50%)

1 (25%)

1 (25%)

2 (50%)

≥10 - <25%

-

-

-

-

≥25 - <50%

-

1 (25%)

1 (25%)

-

≥50%

-

-

2 (50%)

-

II

0

2 (28.6%)

1 (14.3%)

-

1 (14.3%)

>0 - <10%

3 (42.8%)

1 (14.3%)

2 (28.6%)

1 (14.3%)

≥10 - <25%

1 (14.3%)

1 (14.3%)

2 (28.6%)

3 (42.8%)

≥25 - <50%

1 (14.3%)

1 (14.3%)

1 (14.3%)

2 (28.6%)

≥50%

-

2 (28.6%)

2 (28.6%)

-

III

0

5 (83.3%)

2 (33.3%)

-

-

>0 e <10%

-

2 (33.3%)

1 (16.7%)

-

≥10 - <25%

1 (16.7%)

1 (16.7%)

2 (33.3%)

1 (16.7%)

≥25 - <50%

-

1 (16.7%)

2 (33.3%)

4 (66.6%)

≥50%

-

-

1 (16.7%)

1 (16.7%)

  1. Number of positive cases (and percentage) for each ranges of positive cells observed, grouped in different grades, according to Kirpensteijn’s grading system [45]. N: nuclear; M: membranous; C: cytoplasmic.