Effects of ZSTK474, KP372-1 and Rapamycin on induction of apoptosis. Cells were treated with 20 μM ZSTK474 for 2 days, 400 nM KP372-1 for 1 day, 20 μM Rapamycin for 2 days, or vehicle control as described in Materials and Methods. Induction of apoptosis was determined by annexin V/propidium iodide (PI) staining and flow cytometry analysis. Scatter pots with percentages of live, early apoptosis and late apoptosis were indicated at lower left, lower right and upper right quadrants, respectively in A while B demonstrates this data in a bar chart format. This data is representative of two independent experiments.