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Table 1 List of identified sheep serum proteins

From: Proteomic evaluation of sheep serum proteins

SPOT N° (a)

NAME

ACCESSION CODE(b)

Theoretical Mr/pI

Experimental Mr/pI (e)

# peptides MS

# total peptides MS/MS

Mascot SCORE(c)

Orthologue from(d)

1

α 1 Antitripsin

A1AT_SHEEP

45.984/5.83

63.0/4.47

17

2

279 m

 

2

α 1 Antitripsin

A1AT_SHEEP

45.984/5.83

62.8/4.55

14

10

311 e

 

3

α 1 Antitripsin

A1AT_SHEEP

45.984/5.83

63.7/4.40

16

9

249 e

 

4

α 1 Antitripsin

A1AT_SHEEP

45.984/5.83

50.5/4.43

7

2

45 m

 

5

α 1 Antitripsin

A1AT_SHEEP

45.984/5.83

50.4/4.52

9

3

99 m

 

6

α 1B Glycoprotein

A1BG_BOVIN

53.553/5.30

74.1/4.39

16

5

176 m

Bovine

7

α 1B Glycoprotein

A1BG_BOVIN

53.553/5.30

73.3/4.48

9

4

146 m

Bovine

8

α 1 acid Glycoprotein

A1AG_BOVIN

23.182/5.62

46.1/3.35

6

2

82 m

Bovine

9

Amine Oxidase

AOCX_BOVIN

84.756/5.57

90.1/5.10

19

5

161 m

Bovine

10

Apolipoprotein A1

O02762_SHEEP

66.173/4.61

25.2/5.24

18

8

256 m

 

11

Apolipoprotein A1

O02762_SHEEP

66.173/4.61

25.7/5.01

11

3

137 m

 

12

Apolipoprotein A1

O02762_SHEEP

66.173/4.61

26.0/4.80

31

16

650 m

 

13

Apolipoprotein A1

O02762_SHEEP

66.173/4.61

26.2/4.63

13

2

235 m

 

14

Apolipoprotein A1

O02762_SHEEP

66.173/4.61

26.0/5.51

15

8

273 e

 

15

Ceruloplasmin

CERU_SHEEP

119.126/5.50

137.2/5.06

16

5

267 m

 

16

Ceruloplasmin

CERU_SHEEP

119.126/5.50

137.8/5.15

15

7

279 m

 

17

Ceruloplasmin

CERU_SHEEP

119.126/5.50

137.1/5.01

14

6

218 m

 

18

Protein kinase

KAPCA_SHEEP

40.589/8.71

32.5/3.66

11

3

95 m

 

19

Complement component 3

O97941_BOVIN

17.044/5.25

67/9.38

5

2

81 m

Bovine

20

Corticotropin releasing factor

CRFR1_SHEEP

47.558/9.63

93.7/5.46

7

2

62 m

 

21

Fetuin Precursor

FETUA_SHEEP

38.680/5.17

69.7/4.02

7

4

157 m

 

22

Fetuin Precursor

FETUA_SHEEP

38.680/5.17

56.2/4.04

8

3

116 m

 

23

α 1B Glycoprotein

A1BG_BOVIN

53.553/5.30

75.6/4.16

14

6

218 m

Bovine

24

α 1B Glycoprotein

A1BG_BOVIN

53.553/5.30

74.8/4.23

12

6

239 m

Bovine

25

α 1B Glycoprotein

A1BG_BOVIN

53.553/5.30

74.5/4.26

21

13

366 m

Bovine

26

α 1B Glycoprotein

A1BG_BOVIN

53.553/5.30

73.9/4.35

11

2

88 m

Bovine

27

Fibrinogen α chain

FIBA_BOVIN

67.012/6.73

56.0/6.31

6

2

81 m

Bovine

28

Fibrinogen β chain

FIBB_BOVIN

53.339/8.45

56.6/6.00

14

7

175 e

Bovine

29

Fibrinogen β chain

FIBB_BOVIN

53.339/8.45

55.0/6.54

16

6

200 m

Bovine

30

Fibrinogen β chain

FIBB_BOVIN

53.339/8.45

55.0/6.70

11

4

132 m

Bovine

31

Fibrinogen α chain

FIBA_BOVIN

67.012/6.73

74.4/6.96

5

2

90 m

Bovine

32

Fibrinogen α chain

FIBA_BOVIN

67.012/6.73

74.7/6.86

4

2

78 m

Bovine

33

Fibrinogen α chain

FIBA_BOVIN

67.012/6.73

74.0/6.72

11

3

73 m

Bovine

34

Fibrinogen α chain

FIBA_BOVIN

67.012/6.73

74.4/6.78

5

2

61 m

Bovine

35

Fibrinogen α chain

FIBA_BOVIN

67.012/6.73

65.3/6.94

9

2

87 m

Bovine

36

Fibrinogen β chain

FIBB_BOVIN

53.339/8.45

65.3/6.81

17

4

130 m

Bovine

37

Gelsolin

F2YQ13_SHEEP

80.719/5.59

94.4/5.87

13

4

156 m

 

38

Haptoglobin

HPT_BOVIN

44.859/7.83

36.7/6.38

9

4

185 m

Bovine

39

Haptoglobin

HPT_BOVIN

44.859/7.83

36.6/6.56

18

5

222 m

Bovine

40

Haptoglobin

HPT_BOVIN

44.859/7.83

36.6/6.53

17

4

182 m

Bovine

41

Haptoglobin

HPT_BOVIN

44.859/7.83

36.6/6.66

7

2

76 m

Bovine

42

Hemoglobin β

HBB_SHEEP

16.073/6.75

12.7/7.82

23

8

516 m

 

43

Hemoglobin β

HBB_SHEEP

16.073/6.75

12.6/8.24

21

7

428 m

 

44

α globin chain

Q28745_SHEEP

15.178/7.94

12.2/8.70

17

11

306 m

 

45

α globin chain

Q28745_SHEEP

15.178/7.94

12.2/9.72

18

10

491 m

 

46

Ig λ -1 chain

CAA494511

51.564/5.65

41.0/8.69

4

3

141 e

 

47

Ig λ -1 chain

CAA494511

51.564/5.65

41.0/8.82

7

5

158 e

 

48

Ig λ -1 chain

CAA494511

51.564/5.65

41.0/8.97

7

3

137 e

 

49

Ig λ -1 chain

CAA494511

51.564/5.65

51.4/8.65

8

6

150 e

 

50

Ig mu chain

AAA513791

53.545/5.10

80.9/5.31

17

12

319 m

 

51

Ig mu chain

AAA513791

53.545/5.10

80.9/5.45

16

12

314 m

 

52

Apolipoprotein A1

AAI02942

30.276/5.71

26.9/5.68

24

7

399 m

Bovine

 

IgG α λ-chain

AAU45093

11.328/8.48

26.9/5.68

19

10

337 m

 

53

IgG α λ-chain

AAU45093

11.328/8.48

26.6/9.11

14

6

202 m

 

54

IgG α λ-chain

AAU45093

11.328/8.48

26.7/8.27

9

3

113 m

 

55

IgG α λ-chain

AAU45093

11.328/8.48

27.6/6.39

14

5

286 m

 

56

IgG α λ-chain

AAU45093

11.328/8.48

26.4/9.43

11

4

146 m

 

57

IgG α λ-chain

AAU45093

11.328/8.48

51.2/8.78

7

2

98 m

 

58

Ig λ -1 chain

CAA494511

51.564/5.65

51.1/8.69

5

2

68 m

 

59

IgG α λ-chain

AAU45093

11.328/8.48

26.1/9.95

13

2

118 m

 

60

IgG α λ-chain

AAU45093

11.328/8.48

27.1/5.13

7

3

115 m

 

61

Ig mu chain

AAA513791

53.545/5.10

52.4/6.85

8

2

102 m

 

62

α Tripsin Inhibitor Chain H4

ITIH4_BOVIN

101.512/6.22

119.4/5.52

7

2

82 m

Bovine

63

Muscle Endopin 1b

SPA33_BOVIN

46.326/6.05

126.3/4.18

6

2

88 m

Bovine

64

Muscle Endopin 1b

SPA33_BOVIN

46.326/6.05

126.4/4.28

16

4

142 m

Bovine

65

Retynol-Binding protein

RET3_SHEEP

27.992/3.49

22.2/5.59

14

6

287 m

 

66

Serum albumin

ALBU_SHEEP

69.188/5.80

44.3/5.22

13

8

236 m

 

67

Serum albumin

ALBU_SHEEP

69.188/5.80

42.0/5.23

12

6

193 e

 

68

Serum albumin

ALBU_SHEEP

69.188/5.80

39.8/5.53

19

10

359 m

 

69

Serum albumin

ALBU_SHEEP

69.188/5.80

34.7/5.52

17

7

279 m

 

70

Serum albumin

ALBU_SHEEP

69.188/5.80

46.6/5.23

9

4

138 m

 

71

Serum albumin

ALBU_SHEEP

69.188/5.80

34.8/5.95

14

8

258 m

 

72

Serum albumin

ALBU_SHEEP

69.188/5.80

35.6/5.77

15

6

212 m

 

73

Serum albumin

ALBU_SHEEP

69.188/5.80

16.5/4.68

12

5

167 e

 

74

Serum albumin

ALBU_SHEEP

69.188/5.80

16.1/5.05

16

7

222 m

 

75

Serum albumin

ALBU_SHEEP

69.188/5.80

21.8/4.53

15

9

326 m

 

76

Serum albumin

ALBU_SHEEP

69.188/5.80

22.1/4.47

15

10

332 m

 

77

Serum albumin

ALBU_SHEEP

69.188/5.80

23.9/4.41

16

9

335 m

 

78

Serum albumin

ALBU_SHEEP

69.188/5.80

11.2/4.36

11

5

176 e

 

79

Serum albumin

ALBU_SHEEP

69.188/5.80

11.3/4.06

8

2

93 e

 

80

Serum albumin

ALBU_SHEEP

69.188/5.80

9.6/3.93

7

4

119 m

 

81

Serum albumin

ALBU_SHEEP

69.188/5.80

116.4/5.69

7

2

71 m

 

82

Serum albumin

ALBU_SHEEP

69.188/5.80

116.3/5.74

10

2

115 e

 

83

Serum albumin

ALBU_SHEEP

69.188/5.80

93.5/5.53

21

11

353 m

 

84

Serum albumin

ALBU_SHEEP

69.188/5.80

92.8/6.08

19

8

275 m

 

85

Serum albumin

ALBU_SHEEP

69.188/5.80

31.3/6.29

18

6

248 m

 

86

Serum albumin

ALBU_SHEEP

69.188/5.80

31.3/6.55

13

5

173 m

 

87

Serum albumin

ALBU_SHEEP

69.188/5.80

30.8/6.83

14

7

273 m

 

88

Serum albumin

ALBU_SHEEP

69.188/5.80

27.4/6.70

15

6

237 m

 

89

Serum albumin

ALBU_SHEEP

69.188/5.80

25.5/6.94

16

6

254 m

 

90

Serum albumin

ALBU_SHEEP

69.188/5.80

26.4/7.03

15

8

270 m

 

91

Serum albumin

ALBU_SHEEP

69.188/5.80

26.4/7.25

14

5

195 m

 

92

Serum albumin

ALBU_SHEEP

69.188/5.80

25.7/7.16

16

5

219 m

 

93

Serum albumin

ALBU_SHEEP

69.188/5.80

36.5/6.26

13

7

265 m

 

94

Serum albumin

ALBU_SHEEP

69.188/5.80

74.3/3.05

15

7

261 m

 

95

Serum albumin

ALBU_SHEEP

69.188/5.80

27.2/5.40

5

2

75 m

 

96

Serum albumin

ALBU_SHEEP

69.188/5.80

24.7/6.24

8

4

161 m

 

97

Serum albumin

ALBU_SHEEP

69.188/5.80

25.5/6.38

5

2

88 e

 

98

Serum albumin

ALBU_SHEEP

69.188/5.80

24.6/6.69

17

10

369 m

 

99

Serum albumin

ALBU_SHEEP

69.188/5.80

93.5/5.68

14

7

222 m

 

100

Serum albumin

ALBU_SHEEP

69.188/5.80

43.0/4.76

6

2

87 m

 

101

Serum albumin

ALBU_SHEEP

69.188/5.80

49.3/6.46

23

13

456 e

 

102

Serum albumin

ALBU_SHEEP

69.188/5.80

49.4/6.30

21

12

401 m

 

103

Serum albumin

ALBU_SHEEP

69.188/5.80

63.704

15

8

276 m

 

104

Serum albumin

ALBU_SHEEP

69.188/5.80

63.9/6.08

22

13

461 m

 

105

Serum albumin

ALBU_SHEEP

69.188/5.80

150.2/5.64

21

10

362 m

 

106

Transferrin

O18780_SHEEP

66.454/8.29

77.5/6.44

12

4

125 m

 

107

Transferrin

O18780_SHEEP

66.454/8.29

77.3/6.33

13

4

145 m

 

108

Transferrin

O18780_SHEEP

66.454/8.29

76.9/6.54

8

3

142 e

 

109

Transferrin

O18780_SHEEP

66.454/8.29

82.1/6.66

18

8

279 m

 

110

Transferrin

O18780_SHEEP

66.454/8.29

82.8/6.22

6

2

60 m

 

111

Transferrin

O18780_SHEEP

66.454/8.29

82.1/6.32

6

3

117 m

 

112

Transthyretin

TTHY_SHEEP

15.770/5.59

13.0/5.87

15

9

375 e

 

113

Transthyretin

TTHY_SHEEP

15.770/5.59

13.1/5.42

18

14

479 e

 

114

Apolipoprotein AII

APOA2_BOVIN

11.201/7.80

10.0/4.03

5

2

77 m

Bovine

115

VitD Binding Protein

VTDB_BOVIN

53.342/5.36

64.7/4.48

5

2

104 m

Bovine

116

VitD Binding Protein

VTDB_BOVIN

53.342/5.36

64.7/4.56

5

2

46 m

Bovine

117

VitD Binding Protein

VTDB_BOVIN

53.342/5.36

63.2/4.86

4

2

40 m

Bovine

118

VitD Binding Protein

VTDB_BOVIN

53.342/5.36

62.9/4.92

5

2

59 e

Bovine

119

Zn A2 Glycoprotein

ZA2G_BOVIN

33.851/5.11

50.2/3.66

13

7

271 m

Bovine

120

Factor XIIa Inhibitor

F12AI_BOVIN

51.723/6.19

88.4/4.06

6

2

82 e

Bovine

121

Factor XIIa Inhibitor

F12AI_BOVIN

51.723/6.19

88.1/4.12

6

3

119 m

Bovine

122

Factor XIIa Inhibitor

F12AI_BOVIN

51.723/6.19

87.8/4.18

9

3

148 m

Bovine

123

Ig mu chain

AAA513791

53.545/5.10

82.8/4.88

13

3

120 m

 

124

Ig mu chain

AAA513791

53.545/5.10

81.8/5.01

14

5

152 m

 

125

Ig λ -6a light chain

AAB94911

11.718/4.65

24.2/4.87

11

4

135 m

 

126

Ig G heavy chain

AAB37381

35.848/6.09

53.8/6.31

7

2

68 m

Bovine

127

Paraoxonase 1

Q2KIW1_BOVIN

39.841/5.24

46.8/3.89

9

4

137 m

Bovine

128

Paraoxonase 1

Q2KIW1_BOVIN

39.841/5.24

46.6/4.01

8

3

122 e

Bovine

129

Muscle Endopin 1b

SPA33_BOVIN

46.326/6.05

126.6/4.38

10

6

192 m

Bovine

130

Muscle Endopin 1b

SPA33_BOVIN

46.326/6.05

126.4/4.58

11

7

204 m

Bovine

131

Fetuin

FETUA_SHEEP

38.680/5.17

68.1/4.06

8

2

83 m

 

132

Fetuin

FETUA_SHEEP

38.680/5.17

67.7/4.12

10

4

172 m

 

133

Fetuin

FETUA_SHEEP

38.680/5.17

66.2/4.01

12

7

209 m

 

134

Fetuin

FETUA_SHEEP

38.680/5.17

56.2/4.42

11

5

163 m

 

135

Gelsolin

F2YQ13_SHEEP

80.719/5.59

94.4/5.87

10

6

214 e

 

136

Gelsolin

F2YQ13_SHEEP

80.719/5.59

92.8/6.08

13

8

245 e

 

137

Corticotropin

550074A

45.421/6.51

93.6/5.30

17

11

316 m

 

138

Corticotropin

550074A

45.421/6.51

93.9/5.38

10

4

141 m

 
  1. a) Number refer to protein spots indicated in Figures.
  2. b) Accession number from SWISS-PROT or NCBI entry is given.
  3. c) MOWSE score required for significant homology (P < 0.05); "m" indicates MALDI-TOF/TOF-MS; "e" indicates CE-ESI-MS.
  4. d) Sheep protein or homologue to.
  5. e) Values for Mr and pI experimentally determined for each spot according to marker proteins.
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