Differential 2-DE analysis of serum in sheep with RTB. A) 2-DE serum sheep map and expanded view of the gel portions including the deregulated proteins. Spot proteins differing by > 2 fold (P < 0.05) in sheep with RTB compared to healthy sheep (control) were indicated. Area 1: spots identified as Apo A-1; Area 2: spots identified as TTR; Area 3: spots identified as Hp; Area 4: spots identified as EP; Area 5: spots identified as α1BGP. B) Histograms representing log fold change (calculated as a ratio of average density spots in RTB vs average density spots in healthy controls). *: Spots with fold change more than 2 or less than 0.5 with statistically significant P < 0.05.