Effect of adjuvants on the humoral immune response to congopain in mice and cattle

BMC Veterinary Research

Table 1 Computation of percent inhibition of C2 activity

IgG conc. [μg/ml]	Slope of linear plot of residual C2 activity	Slope of linear plot of C2 activity assayed with non- immune IgGs	Percent inhibition of C2 activity
250	4.6078	8.0837	43
125	4.6724	7.1883	35
62.5	5.2348	7.3729	29

The slopes of the linear plots for the residual C2 activity were determined in a spectrofluorometer using excitation at 360 nm and emission at 460 nm. Percent inhibition of C2 activity was determined using the formula: $Percent inhibition of C 2 = \frac{slope (C 2 + non - immune IgG) - slope (C 2 + immune IgG) \times 100}{(C 2 + non - immune IgG)}$
Immune IgGs were obtained from mice or cattle immunized with C2 with adjuvant while non-immune IgGs were obtained from mice or cattle before immunization. The table shows the computation of percent inhibition of C2 activity by IgGs from mice immunized with C2 in Adjuphos™ adjuvant as an example.

ISSN: 1746-6148