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Table 2 Mean and standard deviation of optical density readings for 100 serum samples from two non-infected herds, together with the calculated cut-off value for negativity by ELISA using either whole cell (WC) or recombinant Bhlp29.7 as plate coating antigens with serum and meat juice samples

From: The use of ELISAs for monitoring exposure of pig herds to Brachyspira hyodysenteriae

 

Serum

Meat juice

 

IgG

IgM

IgG

IgM

 

WC

Bhlp29.7

WC

Bhlp29.7

WC

Bhlp29.7

WC

Bhlp29.7

Mean ± SD

0.63 ± 0.1

0.52 ± 0.10

0.68 ± 0.11

0.78 ± 0.09

0.32 ± 0.06

0.68 ± 0.13

0.71 ± 0.09

0.64 ± 0.12

Cut-off value

0.93

0.83

1.0

1.06

0.49

1.08

0.99

0.96