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Table 3 Nucleotide sequence of PCR primers used for the construction of genetically modified clone

From: Evaluation of a genetically modified foot-and-mouth disease virus vaccine candidate generated by reverse genetics

Name

Nucleotide Sequence(5′ → 3′)

Nucleotide Position (nt)

HN2729F

TCCTACACTTCGA CGGTGACGTACCGTAC

2729–2752(E → D)

HN2729R

GTACGGTACGTCACCG TCGAAGTGTAGGA

2729–2752(E → D)

HN3341F

GATTTGTGAAAGTCA A ACCAAAAGACCAAAT

3341–3371(T → K)

HN3341R

ATTTGGTCTTTTGGT T TGACTTTCACAAATC

3341–3371(T → K)

HN3356F

CACCAAAAGACCAAG TCAATGTGCTGGACC

3356–3385(I → V)

HN3356R

GGTCCAGCACATTGA CTTGGTCTTTTGGTG

3356–3385(I → V)

ZP1F

CAACCTACACTTCATGTTCACAGG

2883–2906

ZP2R

AGACGGTCGCTACAACGGAAGTA GTAAG

3610–3659(C → S,S → G,A → T,

TACG GTGACA CC A GC AC GA A C

R → S,V → T,S → N)

ZP3F

G T TC GT GC T GG TGTCAC CGTACTTAC TA

3610–3659(C → S,S → G,A → T,

CTTCCGTTGTAGCGACCGTCT

R → S,V → T,S → N)

ZP4R

TGCTTGTGTCTAGCGTCACTCG

3815–3836

  1. Mutated nucleotides are underlined and in bold and the codon of the mutation effects are indicated in bold. Amino acid changes are shown in brackets.