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Table 2 Nucleotide sequence of PCR primers used for the construction of FMDV O/HN/CHA/93 full-length cDNA clone

From: Evaluation of a genetically modified foot-and-mouth disease virus vaccine candidate generated by reverse genetics

Name

Nucleotide Sequence (5′ → 3′)

Nucleotide Position (nt)

Z1

AGACTAGT taatacgactcactata T7promoter GGGTTG

1–20

AAAGGGGGCGCTAGGGT

 

Z1′

GAGGAGGGGGGGGGGGGGGGTGAAAG

363–388

Z2

CTTTCACCCCCCCCCCCCCCCTCCTC

363–388

Z2′

GAAGCAACAGTGCTGCTACT

721–740

Z3

CGCGCCGTCGCTTGAGGAAG

560–579

Z3′

GGGTCGGAGCTCCTCCTTGATAGA

5433–5456

Z4

AGCCACCTCTTCAGAACGTCTACC

5242–5265

Z4′

ACGCGGCCGCTTTTTTTTTTTTTTTTTTTTT

3′ end

Ztu1

GGAGAGCGAGTCAGATATCTTCTTCACTACA

4221–4251

Ztu1′

TGTAGTGAAGAAGATATCTGACTCGCTCTCC

4221–4251

Ztu2

GAAAACGCCTGAGGCCGCCGCACACTGCATT

2967–2997

Ztu2′

AATGCAGTGTGCGGCGGCCTCAGGCGTTTTC

2967–2997

  1. Restriction enzyme sites introduced during clone construction are boxed. Mutated nucleotides are underlined and in bold.