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Table 3 Results for the chi-squared test and kappa coefficients for the four Map detection methods

From: Evaluation of combined high-efficiency DNA extraction and real-time PCR for detection of Mycobacterium avium subsp. paratuberculosis in subclinically infected dairy cattle: comparison with faecal culture, milk real-time PCR and milk ELISA

  HYDEqPCR Faecal culture Milk ELISA
HYDEqPCR
N   141  
chi-squared   4.275  
P   0.039  
Kappa   0.059  
Milk qPCR
N 91 91 91
chi-squared 0.289 0.841 0.575
P 0.591 0.359 0.448
Kappa -0.027 0.084 -0.022
Milk ELISA
N 91 91  
chi-squared 0.111 5.123  
P 0.739 0.024  
Kappa 0.002 0.107  
  1. n: number of samples.
  2. p ≤0.05 indicates significant association between test results.
  3. Kappa coefficient value of 1.00 indicates perfect agreement and a value of 0.00 indicates that any agreement is no better than chance.