Inflammatory responses to induced infectious endometritis in mares resistant or susceptible to persistent endometritis

Table 1 Oligonucleotide primer sequences for amplification of various equine cytokines, SAA and reference genes

Target (gene)		Primer sequence (5'-3')	Product size (bp)	Source/Accesionnumber
SAA	F	CCT GGG CTG CTA AAG TCA TC	169	[25]
	R	AGG CCA TGA GGT CTG AAG TG
TNFa	F	GGC CCA GAC ACT CAG ATC AT	73	[9]
	R	TTG GGG GTT TGC TAC AAC AT
IL1b3i	F	CAG TCT TCA GTG CTC AGG TTT CTG	84	[9]
	R	CAT TGC CGC TGC AGT AAG T
IL-10i	F	GCT GGA GGA CTT TAA GGG TTA C	76	[9]
	R	CAT CAC CTC CTC CAG GTA AAA
IL-8i	F	CTT TCT GCA GCT CTG TGT GAA G	189	[9]
	R	GCA GAC CTC AGC TCC GTT GAC
β-actin	F	CGT GGG CCG CCC TAG GCA CCA	243	AF035774.1
	R	TTG GCC TTA GGG TTC AGG GGG G
β-glucoronidase	F	GCT CAT CTG GAA CTT TGC TGA TTT T	85	XM001493514.2
	R	CTG ACG AGT GAA GAT CCC CTT TT
GapdH	F	GGG TGG AGC CAA AAG GGT CAT CAT	418	[26]
	R	AGC TTT CTC CAG GCG GCA GGT CAG
IL 6	F	GGA TGC TTC CAA TCT GGG TTC AAT	65	[27]
	R	TCC GAA AGA CCA GTG GTG ATT TT
IL-1ra	F	ACA AAT GTG GCT CCT CCA AG	88	NM_001082525
	R	TTT CAG AGC GTC AGA AGT GC

ISSN: 1746-6148