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Table 1 Oligonucleotide primer sequences for amplification of various equine cytokines, SAA and reference genes

From: Inflammatory responses to induced infectious endometritis in mares resistant or susceptible to persistent endometritis

Target (gene)   Primer sequence (5'-3') Product size (bp) Source/Accesionnumber
SAA F CCT GGG CTG CTA AAG TCA TC 169 [25]
  R AGG CCA TGA GGT CTG AAG TG   
TNFa F GGC CCA GAC ACT CAG ATC AT 73 [9]
  R TTG GGG GTT TGC TAC AAC AT   
IL1b3i F CAG TCT TCA GTG CTC AGG TTT CTG 84 [9]
  R CAT TGC CGC TGC AGT AAG T   
IL-10i F GCT GGA GGA CTT TAA GGG TTA C 76 [9]
  R CAT CAC CTC CTC CAG GTA AAA   
IL-8i F CTT TCT GCA GCT CTG TGT GAA G 189 [9]
  R GCA GAC CTC AGC TCC GTT GAC   
β-actin F CGT GGG CCG CCC TAG GCA CCA 243 AF035774.1
  R TTG GCC TTA GGG TTC AGG GGG G   
β-glucoronidase F GCT CAT CTG GAA CTT TGC TGA TTT T 85 XM001493514.2
  R CTG ACG AGT GAA GAT CCC CTT TT   
GapdH F GGG TGG AGC CAA AAG GGT CAT CAT 418 [26]
  R AGC TTT CTC CAG GCG GCA GGT CAG   
IL 6 F GGA TGC TTC CAA TCT GGG TTC AAT 65 [27]
  R TCC GAA AGA CCA GTG GTG ATT TT   
IL-1ra F ACA AAT GTG GCT CCT CCA AG 88 NM_001082525
  R TTT CAG AGC GTC AGA AGT GC