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Table 3 Dose-dependent effect of SNP and inhibitors on PGE 2 production in canine CCL and CaCL cells

From: Nitric oxide induces cell death in canine cruciate ligament cells by activation of tyrosine kinase and reactive oxygen species

  PGE2 [pg/mL/2 × 106 cells]
Treatment CCL cells CaCL cells Human chondrocytes
Control 35.4 ± 0.1 29.0 ± 0.6 620 ± 10
SNP (0.2 mM) 90.1 ± 1.3* 81.8 ± 2.2* 995 ± 30*
SNP (0.4 mM) 134.2 ± 5.4* 91.6 ± 14.5*  
SNP (0.5 mM) 247.2 ± 16.9* 108.6 ± 10.6* 1636 ± 22*
SNP (1.0 mM) 239.4 ± 12.6* 179.8 ± 8.2* 12790 ± 870*
SNP (0.2 mM) + Genistein (50 μM) 39.5 ± 3.4† 43.1 ± 2.3†  
SNP (0.2 mM) + NS-398 (50 μM) 46.2 ± 2.1† 46.3 ± 3.8†  
  1. Data are the mean ± SD from two separate experiments each performed in triplicates. PGE2 release in pg of 2 × 106 cells into 1 mL medium was measured by enzyme immunoassay. Human chondrocytes served as positive controls. *, P < 0.01 vs. control; †, P < 0.01 vs. SNP (0.2 mM); Dunnett`s multiple comparison test.
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