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Table 3 Dose-dependent effect of SNP and inhibitors on PGE 2 production in canine CCL and CaCL cells

From: Nitric oxide induces cell death in canine cruciate ligament cells by activation of tyrosine kinase and reactive oxygen species

 

PGE2 [pg/mL/2 × 106 cells]

Treatment

CCL cells

CaCL cells

Human chondrocytes

Control

35.4 ± 0.1

29.0 ± 0.6

620 ± 10

SNP (0.2 mM)

90.1 ± 1.3*

81.8 ± 2.2*

995 ± 30*

SNP (0.4 mM)

134.2 ± 5.4*

91.6 ± 14.5*

 

SNP (0.5 mM)

247.2 ± 16.9*

108.6 ± 10.6*

1636 ± 22*

SNP (1.0 mM)

239.4 ± 12.6*

179.8 ± 8.2*

12790 ± 870*

SNP (0.2 mM) + Genistein (50 μM)

39.5 ± 3.4†

43.1 ± 2.3†

 

SNP (0.2 mM) + NS-398 (50 μM)

46.2 ± 2.1†

46.3 ± 3.8†

 
  1. Data are the mean ± SD from two separate experiments each performed in triplicates. PGE2 release in pg of 2 × 106 cells into 1 mL medium was measured by enzyme immunoassay. Human chondrocytes served as positive controls. *, P < 0.01 vs. control; †, P < 0.01 vs. SNP (0.2 mM); Dunnett`s multiple comparison test.