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Table 1 Dose-dependent effect of SNP with/without inhibitors on bcl-2 protein level in canine CCL and CaCL cells

From: Nitric oxide induces cell death in canine cruciate ligament cells by activation of tyrosine kinase and reactive oxygen species

  bcl-2 [ng/mg protein]
  CCL cells (n = 6) CaCL cells (n = 6)
Control 6.6 ± 0.3 7.0 ± 0.4
SNP (0.3 mM) 6.5 ± 0.1 5.1 ± 0.3*
SNP (0.5 mM) 5.5 ± 0.3* 4.9 ± 0.2*
SNP (1 mM) 2.8 ± 0.2** 3.9 ± 0.1**
SNP (0.5 mM) + Genistein (50 μM) 8.8 ± 0.2†† 10.9 ± 0.4††
SNP (0.5 mM) + Uric acid (0.5 mM) 7.8 ± 0.1†† 7.9 ± 0.1††
SNP (0.5 mM) + Taxifolin (50 μM) 7.6 ± 0.3† 6.7 ± 0.4†
SNP (0.5 mM) + PTIO (5 μM) 5.8 ± 0.2 5.1 ± 0.2
  1. Data represent the means and standard deviations from two separate experiments, n refers to the number of donors, and each performed in triplicates. Bcl-2 protein was measured by enzyme immunoassay. *, P < 0.05, **, P < 0.01 vs. control; †, P < 0.05, ††, P < 0.01 vs. SNP (0.5 mM); Dunnett`s multiple comparison test.