Figure 2From: Nitric oxide induces cell death in canine cruciate ligament cells by activation of tyrosine kinase and reactive oxygen speciesApoptotic fraction in canine cruciate ligament cells. Canine CCL and CaCL cells were stimulated with indicated concentrations of SNP for 18 h. Apoptotic cells were measured by FITC-annexinV/propidium iodide double stained flow cytometry. The stacked bar graphs are divided into two categories: patterned graphs (square with upper right to lower left fill) and (light shaded square) indicate the early apoptotic fractions detected as cells stained annexin V positive and propidium iodide negative, plain-colored graphs (dark gray square and light gray square) indicate the end stage apoptosis and death detected as cells stained annexin V and propidium iodide positive. The graphs data represent the mean ± SD from at least three separate experiments of four different cell donors, each performed in triplicates. * P < 0.05, ** P < 0.01 for combined (early and late) apoptotic fractions of CCL or CaCL treated with SNP at each indicated concentration vs. control without SNP treatment.Back to article page