Skip to main content

Table 6 Primers and probes for multiplex real-time RT-PCRa

From: One-step multiplex real time RT-PCR for the detection of bovine respiratory syncytial virus, bovine herpesvirus 1 and bovine parainfluenza virus 3

Viruses

Primers/probes

Sequences (5'-3')

BPI3 i

BPI3euro Forward

GGTAGGAGCACCTCCACGATT

 

BPI3euro Reverse

GCTCCAAGGCATGCTGGATA

 

BPI3euroMGB

VIC-AAGATCTTGTTCACACATTC-MGB-NFQ

BPI3 ii

BPI3 Forward2

TGATTGGATGTTCGGGAGTGA

 

BPI3Reverse2

AGAATCCTTTCCTCAATCCTGATATACT

 

BPI3Fam

FAM-TACAATCGAGGATCTTGTTCA-MGB-NFQ

BRSV [14]

BRSVn Forward

GGTCAAACTAAATGACACTTTCAACAAG

 

BRSVn Reverse

AGCATACCACACAACTTATTGAGATG

 

BRSVLNACyan500

Cyan500-TAGTACAGGTGACAA+CA+T+TG-BBQ

BoHV-1 [15]

gB Forward

TGTGGACCTAAACCTCACGGT

 

gB Reverse

GTAGTCGAGCAGACCCGTGTC

 

gBCy5

Cy5-AGGACCGCGAGTTCTTGCCGC-BHQ2

β-actin

Bac1F_uni

GACAGGATGCAGAARGAGATCAC

 

Bac2R_uni

TCCACATCTGCTGGAAGGTG

 

β-actin FAM-MGB

FAM-TGAAGATCAAGATCATCG-MGB-NFQ

  1. a All the primers and probes are obtained from Applied Biosystems (Warrington, UK) except BRSV LNAcyan500 probe (TIB Molbiol,Berlin, Germany) and gBCy5 probe (Eurofins MWG Operon, Germany).
  2. Abbreviations: BHQ black hole quencher, MGB-NFQ minor groove binding-non fluorescent quencher, LNA locked nucleic acid, BBQ Blackberry quencher.