Skip to main content

Table 2 Estimates (95% lower, upper confidence levels) of mean cut-off Cp values after amplification of a dilution of titrated virus in uniplex and multiplex real time RT-PCR for a test sensitivity of 97%a

From: One-step multiplex real time RT-PCR for the detection of bovine respiratory syncytial virus, bovine herpesvirus 1 and bovine parainfluenza virus 3

   Mean Cut-off Cp value
Virus Copy number Uniplex RTPCR Multiplex RTPCR
BRSV 103 32.9 (32.1, 33.6) 33.0 (32.2, 33.7)
  102 35.4 (34.5, 36.1) 35.4 (34.6, 36.1)
  10 37.7 (36.8, 38.5) 37.7 (36.9, 38.5)
BoHV-1 103 31.4 (31.0, 31.9) 31.4 (31.0, 31.9)
  102 35.2 (34.8, 35.8) 35.2 (34.8, 35.8)
  10 37.1 (36.6, 37.6) 37.1 (36.6, 37.6)
BPI3 i 103 31.7 (31.2, 32.4) 32.5 (31.9, 33.1)
  102 34.4 (33.9, 35.1) 35.2 (34.6, 35.8)
  10 39.4 (39.0, 40.2) 40.2 (39.7, 41.0)
BPI3 ii 103 32.4 (32.2, 32.8) 32.8 (32.6, 33.1)
  102 34.7 (34.4, 35.0) 35.1 (34.9, 35.4)
  10 38.4 (38.2, 38.8) 38.9 (38.7, 39.2)
  1. a For each virus, a 10-fold dilution series is shown with the mean Cp values. The lowest level of detection is shown, as are the two 10-fold dilutions immediately prior to the lowest level of detection. Each dilution series was tested four times in triplicate. The estimates of the mean cut-off Cp value for a test sensitivity of 97% and corresponding 95% confidence intervals were obtained using a bootstrap sampling procedure (see text for detail).
\