Table 2 Estimates (95% lower, upper confidence levels) of mean cut-off Cp values after amplification of a dilution of titrated virus in uniplex and multiplex real time RT-PCR for a test sensitivity of 97%a

BRSV

10³

32.9 (32.1, 33.6)

33.0 (32.2, 33.7)

10²

35.4 (34.5, 36.1)

35.4 (34.6, 36.1)

10

37.7 (36.8, 38.5)

37.7 (36.9, 38.5)

BoHV-1

10³

31.4 (31.0, 31.9)

31.4 (31.0, 31.9)

10²

35.2 (34.8, 35.8)

35.2 (34.8, 35.8)

10

37.1 (36.6, 37.6)

37.1 (36.6, 37.6)

BPI3 i

10³

31.7 (31.2, 32.4)

32.5 (31.9, 33.1)

10²

34.4 (33.9, 35.1)

35.2 (34.6, 35.8)

10

39.4 (39.0, 40.2)

40.2 (39.7, 41.0)

BPI3 ii

10³

32.4 (32.2, 32.8)

32.8 (32.6, 33.1)

10²

34.7 (34.4, 35.0)

35.1 (34.9, 35.4)

10

38.4 (38.2, 38.8)

38.9 (38.7, 39.2)