A novel bead-based assay to detect specific antibody responses against Toxoplasma gondii and Trichinella spiralissimultaneously in sera of experimentally infected swine

Table 3 Inter-test agreement between assays calculated by McNemar's and Cohen's Kappa analysis

	McNemar's test Yates correction	Cohen's Kappa
Test comparison	P	κ*	95% CI
BBA-TOX vs. E1-TOX	< 0.001	0.676	0.607-0.744
BBA-TOX vs. E2-TOX	0.01	0.802	0.746-0.857
BBA-TOX vs. E3-TOX	0.06	0.932	0.900-0.966
E1-TOX vs. E2-TOX	< 0.001	0.752	0.690-0.814
E1-TOX vs. E3-TOX	< 0.001	0.723	0.658-0.787
E2-TOX vs. E3-TOX	0.233	0.797	0.740-0.853
BBA-TRI vs. E-TRI	< 0.001	0.880	0.835-0.925

Inter-test agreement calculations comparing between dichotomized results of tests. P, probability; κ, Cohen's Kappa value; 95% CI, interval at a 95% confidence; BBA-TOX, T. gondii antigen coupled bead-based assay; E1-TOX, RIVM in-house T. gondii ELISA; E2-TOX, Safepath T. gondii ELISA; E3-TOX, ID-VET T. gondii ELISA; BBA-TRI, T. spiralis antigen coupled bead-based assay and E-TRI, Safepath T. spiralis ELISA. * indicates that all values were statistically significant (P < 0.001)

ISSN: 1746-6148