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Table 2 Infection status based specifications of bead-based assay and ELISA tests calculated by ROC analysis

From: A novel bead-based assay to detect specific antibody responses against Toxoplasma gondii and Trichinella spiralissimultaneously in sera of experimentally infected swine

 

ROC analysis

     

Maximum Youden Index

test

AUC

SE

P

95% CI

Cut-off

Se

Sp

BBA-TOX

0.911

0.015

< 0.001

0.881-0.940

13.90

0.86

0.96

E1-TOX

0.837

0.018

< 0.001

0.800-0.873

0.369

0.64

0.95

E2-TOX

0.865

0.018

< 0.001

0.829-0.901

0.174

0.76

0.93

E3-TOX

0.930

0.013

< 0.001

0.904-0.956

26.90

0.84

0.99

BBA-TRI

0.855

0.018

< 0.001

0.819-0.890

4.65

0.68

1.00

E-TRI

0.879

0.016

< 0.001

0.849-0.912

0.018

0.72

0.95

  1. ROC analyses of a T. gondii and T. spiralis bead-based assay, three T. gondii and one T. spiralis ELISAs using responses of porcine serum samples from an experimental infection calculated against their infection status. AUC, area under the curve; SE, standard error of AUC; P, P-value; 95% CI, interval at a 95% confidence; Se, sensitivity; Sp, specificity; BBA-TOX, T. gondii antigen coupled bead-based assay; E1-TOX, RIVM in-house T. gondii ELISA, E2-TOX: Safepath T. gondii ELISA, E3-TOX, ID-VET T. gondii ELISA; BBA-TRI, T. spiralis antigen coupled bead-based assay and E-TRI, Safepath T. spiralis ELISA