A novel bead-based assay to detect specific antibody responses against Toxoplasma gondii and Trichinella spiralissimultaneously in sera of experimentally infected swine

Table 1 Non specific binding responses of T.gondii and T. spiralis negative serum sets and conjugate alone

Serum	Bead identification	n	Min. response (MFI)	Max. response (MFI)	Mean response (MFI)	SE
Buffer	REF	98	300	600	500	5
	TOX	98	3,900	4,700	4,300	19
	TRI	98	3,200	3,900	3,500	17
(T. gondii or T. spiralis) negative field sera	REF	947	400	491,500	53,700	2,200
	TOX	932	4,200	269,200	47,900	1,100
	TRI	945	3,400	505,500	75,300	2,300
Experimental infection negative control sera	REF	36	1,100	1,900	4,100	600
	TOX	36	4,900	45,500	21,500	1,900
	TRI	36	4,200	34,800	10,400	1,000

Minimum, maximum and mean response signals of reference (REF), T. gondii (TOX) and T. spiralis (TRI) coupled beads after incubation of buffer, negative field sera (without positive responders in T. gondii ELISA) and sera of the negative control animals of the experimental infection. n, number of tested sera; SE, standard error

ISSN: 1746-6148