Table 2 Characteristics and performance data of the primers used for reference gene-stability measure M and quantitative real-time PCR analysis
Gene | Forward primer (from 5' to 3') | PCR product | NCBI GenBank | Slope | R2 | Efficiency | M |
|---|---|---|---|---|---|---|---|
Reverse primer (from 5' to 3') | |||||||
Reference genes | |||||||
ACTB | ACTTGCGCAGAAAACGAGAT | 120 | AY141970 | -0.30 | 0.99 | 1.99 | 0.039 |
CACCTTCACCGTTCCAGTTT | |||||||
SDHA | GCAGAACCTGATGCTTTGTG | 185 | NM_174178 | -0.24 | 0.99 | 1.74 | 0.048 |
CGTAGGAGAGCGTGTGCTT | |||||||
ATP5B | GGACTCAGCCCTTCAGCGCC | 229 | NM_175796.2 | -0.16 | 0.99 | 1.44 | 0.039 |
GCCTGGTCTCCCTGCCTTGC | |||||||
RPS9 | GTGAGGTCTGGAGGGTCAAA | 108 | BC148016 | -0.31 | 0.99 | 2.04 | 0.040 |
GGGCATTACCTTCGAACAGA | |||||||
PPIA | GGCAAATGCTGGCCCCAACACA | 87 | NM_178320.2 | -0.34 | 0.99 | 2.13 | 0.034 |
AGTACCACGTGCTTGCCATCCA | |||||||
RPL12 | CACCAGCCGCCTCCACCATG | 84 | NM_205797.1 | -0.35 | 0.99 | 2.25 | 0.036 |
CGACTTCCCCACCGGTGCAC | |||||||
Target genes | |||||||
ACADM | GCGAGTACCCTGTCCCATTA | 243 | NM_001075235 | -0.29 | 0.99 | 1.93 | |
CCTCAGTCATTCTCCCCAAA | |||||||
ACOX1 | CCATTGCCGTCCGATACAGT | 99 | BC102761 | -0.27 | 0.96 | 1.88 | |
GTTTATATTGCTGGGTTTGATAATCCA | |||||||
ALDH9A1 | CAGGATTCGGCAGAGAGAAC | 229 | NM_001046423 | -0.28 | 0.99 | 1.90 | |
TGAGCCATGAAGAGCATCAC | |||||||
BBOX1 | TCCAGCTGCCTACTCTGGAT | 292 | BC149884.1 | -0.28 | 0.99 | 1.91 | |
AGCTGAACCTTACCCCAGGT | |||||||
CD36 | GCATTCTGAAAGTGCGTTGA | 179 | BC103112 | -0.28 | 0.98 | 1.91 | |
CGGGTCTGATGAAAGTGGTT | |||||||
CPT1A | CAAAACCATGTTGTACAGCTTCCA | 111 | FJ415874 | -0.32 | 0.99 | 2.09 | |
GCTTCCTTCATCAGAGGCTTCA | |||||||
HMGCS2 | GCCCAATATGTGGACCAAAC | 209 | NM_001045883 | -0.29 | 0.99 | 1.96 | |
ATGGTCTCAGTGCCCACTTC | |||||||
PPARA | GGTGGAGAGTTTGGCAGAACCAGA | 168 | BT020756.1 | -0.23 | 0.99 | 1.70 | |
TCCCACTGCCCAGCTCCGATC | |||||||
SLC22A5 | CACAGTGGTCAGGAACATGG | 181 | BC105377 | -0.28 | 0.99 | 1.89 | |
AATGGTGTCTGGGAGTGGAG | |||||||
SLC27A1 | CTGAAGGAGACCTCCACAGC | 208 | NM_001033625.2 | -0.30 | 0.99 | 1.99 | |
GTGGTACAGGGGCAGACAGT | |||||||
TMLHE | TGGCAGGACACTGCTAGTTG | 222 | NM_001076064.1 | -0.31 | 0.99 | 2.05 | |
GACAGCCCGGTCATAGTTGT |