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Figure 6 | BMC Veterinary Research

Figure 6

From: Porcine intestinal epithelial barrier disruption by the Fusariummycotoxins deoxynivalenol and T-2 toxin promotes transepithelial passage of doxycycline and paromomycin

Figure 6

Passage of doxycycline (a) and paromomycin (b) across a differentiated IPEC-J2 cell monolayer, measured as the concentration in the basolateral compartment. Cells were grown for 21 days on Transwell® collagen-coated PTFE filters (0.33 cm2, 0.4 μm). Next, at day 0, cells were incubated for 72 h with different concentrations of DON (untreated inserts, inserts treated with 0.5, 1, 5 or 10 μg/ml), during which the last 24 h a non-cytotoxic concentration of doxycycline and paromomycin was added to the apical compartment (n = 3, mean + stdev). Significant differences in passage have been marked with * (P < 0.05), ** (P < 0.01) or *** (P < 0.001) according to the significance level.

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