Table 1 Primers for canine reverse transcriptase polymerase chain reactions
Primers | Primer sequences | Tm° |
|---|---|---|
Canine Survivin F | 5′- GAA GGC TGG GAG CCA GAT GAT G -3′ | 66.4 |
Canine Survivin R | 5′- CGC ACT TTC TTT GCG GTC TC -3′ | 62.4 |
Canine Cyclin D1 F | 5′- GTC TGC GAG GAG CAG AAG T -3′ | 62.3 |
Canine Cyclin D1 R | 5′- GAG GAA GTG CTC GAT GAA GT -3′ | 60.6 |
Canine BCL-2 F | 5′- GAG CAG CCA CAA CCG GAG AGT C -3′ | 68.3 |
Canine BCL-2 R | 5′- CGG ATC TTT ATT TCA CGA GGC AC -3′ | 62.8 |
Canine MCL-1 F | 5′- CAA CCA CGA GAC AGC CTT CCA AG -3′ | 62.6 |
Canine MCL-1 R | 5′- CAC TGA AAA CAT GGA CAA TCA C -3′ | 58.9 |
Canine 18s F | 5′- AAA TCC TTT AAC GAG GAT CCA TT -3′ | 57.4 |
Canine 18s R | 5′- AAT ATA CGC TAT TGG AGC TGG A -3′ | 58.9 |