Effects of LLL12 on the proliferation of canine OS cell lines and normal osteoblasts. Canine OS cell lines (Abrams, OSA 8, OSA 16 and D17) and normal canine osteoblasts were treated with vehicle or LLL12 for 24 hours. Proliferation was analyzed using the CyQUANT® cell proliferation assay kit. Proliferation values are listed as a percentage of DMSO control. Experiments were performed in triplicate and repeated three times. For each cell line, there was a significant decreasing trend in cell proliferation with dose of LLL12 (p < 0.001).