Neuron-like cells characterization. After 28 days of neurogenic induction, a neuron-like morphology was visible under phase contrast microscopy. A) Neuron-like cells with triangular, round and cone-shaped morphology; B) Bi-polar neuron-like morphology; C) Network among neuron-like cells; D) Long axon of a Golgi neuron;x 100 magnification; E) RT-PCR analysis of neuronal specific markers:nestin, N200, β-tubulin III, GFAP and NT3 transcript expression in neuron-like cells after differentiation were observed. The H2A gene was used as an internal control for the reverse transcription-polymerase chain reaction. MW-molecular weight 1 kb plus.