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Figure 2 | BMC Veterinary Research

Figure 2

From: Canine muscle cell culture and consecutive patch-clamp measurements - a new approach to characterize muscular diseases in dogs

Figure 2

Immunofluorescence of canine myotubes. A, C: immunofluorescence staining of differentiated, multinucleated canine myotubes positive for muscle-specific intermediate filament desmin, primary antibody: mouse anti-Desmin, secondary antibody: goat anti-mouse IgG, Cy3 labelled. B, D: 4′,6-Diamidino-2-phenylindol nucleus staining (=DAPI) demonstrating multiple nuclei of the myotubes (in 60–80% of the cells). E, F: canine fibrocytes culture as negative control, (A, B 400-fold magnification; C, D, E, F 200-fold magnification; bar = 100 μm).

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BMC Veterinary Research

ISSN: 1746-6148

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