In vitro amplification of ovine prions from scrapie-infected sheep from Great Britain reveals distinct patterns of propagation

Table 5 Amplification of MRI, CH1641 and atypical scrapie strains using enhanced PMCA methods

n = number of animals tested. Poly(A) was added to the amplification reactions and the amplifications were carried out for 4 rounds. All analyses were in duplicate except for atypical scrapie samples amplified in ARQ and AHQ substrates which were analysed in single reactions. +/− symbols show the amplification outcome for each animal.

ISSN: 1746-6148