In vitro amplification of ovine prions from scrapie-infected sheep from Great Britain reveals distinct patterns of propagation

Table 2 PMCA-amplification of infected flock/farm mates of ARQ/ARQ scrapie-infected sheep

Farm^*	Sheep ID	Breed	Age at death	PMCA substrates^#
Farm^*	Sheep ID	Breed	Age at death	VRQ/VRQ	ARQ/ARQ
12	913/05	Mule	5y 0 m	+	+
12	SS0-560272	Suffolk Cross	4y	+	+
15	1230/04	Suffolk Cross	2y 4 m	+	+
	709/02	Cheviot/Shetland	5y	+	+
	768/02	Cheviot/Shetland	4y	+	+
	857/04	Cheviot/Shetland	7y 3 m	+	+
	858/04	Cheviot/Shetland	3y 3 m	+	+
	872/04	Cheviot/Shetland	7y 3 m	+	+
13	960/06	White Face Dartmoor	7y	+	+
	703/07	White Face Dartmoor	6y	+	+
	912/07	White Face Dartmoor	7y	+	+
11	297/97	Finn Dorset	not known	-	+
	210/03	Warborough	2y 2 m	+	+
	209/03	Warborough	5y 1 m	-	+
	1583/97	Finn Dorset	4y	-	+
	1685/97	Finn Dorset	4y	-	+
	1002/03	Finn Dorset Cross	7y	-	+
	1158/00	Finn Dorset	6y	-	+
	57/99	Finn Dorset	5y 1 m	-	+

^*Farms 12, 15, 13 and 11 were located in Gloucestershire, Aberdeenshire, Devon and Oxfordshire respectively. Samples were analysed after 4 rounds of PMCA.
^#Each sample was amplified twice using a single substrate of each genotype and a positive sample was recorded when positive in at least one replicate.

ISSN: 1746-6148