In vitro amplification of ovine prions from scrapie-infected sheep from Great Britain reveals distinct patterns of propagation

Table 1 In vitro PMCA amplification of sources of GB scrapie

Sheep ID	PrnP genotype	Farm	Geographic Location^*	Age at death	Amplification in substrates
Sheep ID	PrnP genotype	Farm	Geographic Location^*	Age at death	VRQ	ARQ
1597/98	VRQ/VRQ	1	E. England	2y 7 m	+	-
322/97		2	Wales	2y 0 m	+	-
1563/02		3	W. England	2y 8 m	+	-
836/03		4	S. England	2y 2 m	+	-
1276/02		5	Wales	2y 0 m	+	ND^#
2413/98		6	W. England	2y 2 m	+	ND
456/03	ARQ/VRQ	7	N. England	3y 0 m	+	-
455/03		7	N. England	6y 0 m	+	-
615/03		7	N. England	5 y 0 m	+	-
226/03		7	N. England	5y 0 m	+	-
ss2-022278		8	W. England	not known	+	-
219/06	ARH/VRQ	17	W. England	2y 0 m	+	+
252/06		17	W. England	2y 0 m	+	+
198/06		17	W. England	2y 0 m	+	+
216/06		17	W. England	2y 0 m	+	+
989/02	ARR/VRQ	9	S. England	8y 0 m	+	ND
ss4 506045		10	N. England	not known	+	-
ss4-506228		not known	not known	not known	+	-
297/97	ARQ/ARQ	11	S. England	not known	-	+
ss4-012265		10	N. England	not known	-	+
913/05		12	W. England	5y 0 m	+	+
522		not known	not known	not known	ND	+
960/06		13	W. England	7y 0 m	+	+
1341/04		14	E. England	5y 5 m	-	+
1230/04		15	Scotland	2y 4 m	+	+
284/97	AHQ/AHQ	11	S. England	not known	-	+
1091/01	AHQ/AHQ	16	Midlands	4y 8 m	-	+
217/06	ARH/ARH	17	W. England	1y 0 m	+	+
429/06	ARH/ARH	17	W. England	2y 0 m	+	+
1731/03	ARH/ARQ	18	N. England	1y 6 m	+	+
1741/03		18	N. England	1y 2 m	+	+
1952/04		19	W. England	3y 0 m	+	+
936/05		20	Wales	4y 0 m	+	+
708/02		15	Scotland	5y 0 m	+	+
399/04		15	Scotland	3y 0 m	+	+
462/04		15	Scotland	2y 0 m	+	+
915/07		13	S. England	1y 6 m	+	+

^*S. England: South of England, N. England: North of England, W. England: Welsh borders and West Country, E. England: East of England. All samples were analysed in duplicate after four rounds of PMCA and those marked + were positive in at least one replicate. ^#ND, not done.

ISSN: 1746-6148