Figure 6

Western immunoblot of medulla samples from the L-type BSE donor animal and the four recipients using four different antibodies. A: mAb SHA31, B: mAb 6H4, C: mAb F99, D: mAb P4. Samples were loaded neat and at dilutions indicated in the key. Lane Key: M Molecular mass marker. 1 Donor neat. 2 Donor 1:20. 3 Donor 1:40. 4 L1 neat. 5 L1 1:10. 6 L2 neat. 7 L2 1:10. 8 L3 neat. 9 L3 1:20. 10 L4 neat. 11 L4 1:10. 12 C-type BSE control neat. 13 C-type BSE control 1:20. 14 C-type BSE control 1:40. 15 Standard bovine C-type BSE control 1:40. 16 Standard ovine scrapie control 1:2.