CXCL7 expression in CTVT under IL-1β, TGF-β and IL-6 stimulation. (A) Levels of mRNA expression of CXCL7 after freshly prepared P-phase CTVT cells were treated with IL-1β, TGF-β and/or IL-6 (90 ng/mL) for 12 h, measured by real-time RT-PCR. The amounts of mRNA are expressed relative to the amount of β-actin mRNA in each sample and are shown as the mean ± S.D. (B) 1 μg/ml of anti-IL-6 antibody was adding into the TIL/CTVT cocultured transwell system. TIL cells (0.3 × 106 cells/well) were seeded in the upper chamber and the lower chamber containing CTVT cells (1 × 106 cells/well). After 12 h incubation, the CXCL7 gene expression in the CTVT cells was evaluated. Significant differences in the various stimulators are expressed as ** (P < 0.01) as compared with CTVT alone.