R-phase TIL inhibited the expression of CXCL7 in P-phase CTVT cells. A coculture system was analyzed using a Corning transwell system, in which TIL cells (0.1–1×106 cells/well) were seeded in the upper chamber, with a 0.4-μm-pore filter between that and a lower chamber containing CTVT cells (1×106 cells/well). (A) After 12 h of incubation, the gene expression of CXCL7 in the CTVT cells was evaluated. (B) CXCL7 protein expression was also analyzed after 24-h incubation. Significant differences in the various stimulators are expressed as * (P < 0.05) and ** (P < 0.01) as compared with CTVT alone. The results represent the mean ± S.D. of three independent experiments.