CXCL7 expression is concordant with the CTVT progression phase. (A) Levels of mRNA expression of CXCL7 from CTVT tumor masses (n = 4) measured by real-time RT-PCR. The amounts of mRNA are expressed relative to the amount of β-actin mRNA in each sample and are shown as the mean ± S.D. (**, P < 0.01). (B) Western blot analysis of CXCL7 protein expressed in CTVT. CXCL7 proteins were recognized by polyclonal rabbit antibody against canine CXCL7 in the lysates of CTVT tumor masses. Actin was used as the internal control.