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Table 2 Concentration of growth factors in plasma, supernatants of PC portions at 3 and 12 hours

From: Evaluation of the effect of calcium gluconate and bovine thrombin on the temporal release of transforming growth factor beta 1 and platelet-derived growth factor isoform BB from feline platelet concentrates

Activating substance

Variable

Blood component

Plasma

PC-A (3 h)

PC-A (12 h)

PC-B (3 h)

PC-B (12 h)

Calcium gluconate

TGF-β1 (ng/mL)

7.99 (1.59) a

23.97 (2.03) b

24.79 (2.22) b

26.03 (2.23) b

25.70 (2.43) b

 

PDGF-BB (pg/mL)

365.89 (91.02) a

1571.56 (204.5) b

1420.32 (139.4) b

1552.23 (188.1) b

1400.75 (131.5) b

 

Total protein (mg/mL)

52.93 (1.15) a

44.88 (0.93) b

46.70 (1.36) b

46.21 (1.54) b

46.67 (1.71) b

 

TGF-β1 (ng/mg of TP)

0.15 (0.03) a

0.53 (0.04) b

0.53 (0.05) b

0.57 (0.05) b

0.56 (0.52) b

 

PDGF-BB (pg/mg of TP)

6.91 (1.82) a

35.02 (4.12) b

30.41 (2.50) b

33.59 (3.66) b

30.01 (3.08) b

Bovine thrombin

TGF-β1 (ng/mL)

7.99 (1.59) a

23.98 (2.43) b

28.22 (2.59) b

30.56 (4.47) b

35.74 (4.18) b

 

PDGF-BB (pg/mL)

365.89 (91.02) a

1681.15 (188.3) b

1687.40 (138.8) b

1847.15 (208.3) b

1683.16 (122.7) b

 

Total protein (mg/mL)

52.93 (1.15) a

42.29 (1.37) b

45.05 (2.25) b

43.20 (2.35) b

44.92 (2.15) b

 

TGF-β1 (ng/mg of TP)

0.15 (0.03) a

0.57 (0.06) b

0.65 (0.07) b

0.69 (0.08) b

0.79 (0.09) b

 

PDGF-BB (pg/mg of TP)

6.91 (1.82) a

39.75 (4.38) b

37.45 (3.49) b

42.77 (5.29) b

37.47 (3.11) b

  1. TGF-β1= transforming growth factor beta 1, PDGF-BB= platelet-derived growth factor type BB. Data presented as means (standard error). Different letters denote statistically significant differences between rows (Plasma vs. PCs) by SNK test (P<0.05).