Figure 7
From: Identification of myeloid derived suppressor cells in the peripheral blood of tumor bearing dogs
Purified CD11blowCADO48Alow cells from in vitro differentiated myeloid cells suppressed the proliferation of T and B lymphocytes. Bone marrow cells were differentiated in either GM-CSF (Media) or GM-CSF with canine melanoma tumor-conditioned media for 5 days. Cells were purified and co-cultured at a 1:5 ratio with responder canine splenocytes and the (a) proliferation of cells determined. A cytospin (b) of purified cells shows a mixed population of myeloid and granulocytic precursor cells demonstrating monocytic, granulocytic and ring-shaped nuclei are present in the CD11blow/CADO48Alow populations of both media and melanoma cells. Photomicrograph of the cytospin is at 60x magnification.