Table 1 Genomic and cDNA primers and their locations for PKLR in domestic cats
From: Erythrocyte Pyruvate Kinase Deficiency mutation identified in multiple breeds of domestic cats
gDNA Primer | 5'-3' Sequence | gDNA Primer | 5'-3' Sequence |
|---|---|---|---|
PKLRex2F | CGGTACTTGCTCCACAGGAT (chrF1:88325815–34)* | PKLRex2R | GTGGCGATGATGCAGGTACT (chrF1:88326141–60) |
PKLRex5F | CGAACACCGTGTGGGTAGACTA (derived from cDNA) | PKLRex6R | GGAGGCAAACACGATGTCTACC (chrF1:88327517–38) |
PKLRex6F | TCGTGTTTGCCTCCTTTG (chrF1:88327525–42) | PKLRex10R | AGACCTCTCGGAACAGGTG (chrF1:88329412–30) |
PKLRex9F | TCAAGTGCTGTGCTGCTG (chrF1:88328580–97) | PKLR3utrR | TGGCCAGTGCTTAATTGG (chrF1:88330288–305) |
cDNA Primer | 5'-3' Sequence | cDNA Primer | 5'-3' Sequence |
PKLR_cD_Ex2F | GGGTACCTACGGCGGGTCAG (chrF1:88325991–6010) | PKLR_cD_Ex7R | CTTCTCAGGTGGGATCTCGAT (chrF1:88327825–45) |
PKLR_cD_Ex6F | TCGTGTTTGCCTCCTTTGTGCG (chrF1:88327525–42) | PKLR_cD_Ex10R | AGACCTCTCGGAACAGGTG (chrF1:88329412–30) |
PKLR_cD_Ex8F | GAGACGAGCGATGTAGCG (chrF1:88328256–73) | PKLR_3UTR | CGTGAAATGGAGCAGGGAAGG (chrF1:88330247–67) |
5' Race primer | 5'-3' Sequence | 5' Race primer | 5'-3' Sequence |
AUAP | GGCCACGCGTCGACTAGTAC | PKLR_5'_ex2/3R | CCGGCCCAATGGTGGCG |