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Figure 2 | BMC Veterinary Research

Figure 2

From: Dolphin Morbillivirus and Toxoplasma gondii coinfection in a Mediterranean fin whale (Balaenoptera physalus)

Figure 2

Molecular analyses yielded a positive result for Morbillivirus -specific genome sequences. RT-PCR targeting Morbillivirus nucleoprotein (N) gene gave positive results from the whale's spleen (lane 2), liver (lane 3), and lung (lane 4), while the heart was negative (lane 1); positive and negative controls (C + and C-, respectively) are also shown in figure A along with the DNA ladder marker. Each result was confirmed by means of both sequence and RFLP: the latter is shown in figure B with the positive reaction for spleen (lane 1), liver (lane 2), and lung (lane 3). Immunohistochemistry (IHC) using a commercially available mouse monoclonal antibody (MoAb) solution (1:500) against canine distemper virus (CDV) nucleoprotein antigen (VMRD Inc), which recognizes the same epitope from different members of the Morbillivirus genus (including DMV) was negative, possibly due to bad tissue preservation.

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