Effect of challenge of pigs previously immunised with inactivated vaccines containing homologous and heterologous Mycoplasma hyopneumoniae strains

Table 4 Percentage of seropositive pigs and inhibition percentage (IP) in each group.

Age of pigs	Treatment group
	A	B	C	D	E	F
D7	0	0	0	0	0	0
D28	0^A,C (35.5)^a	17^A (36.2)^a	89^B (54.9)^b	9^A (32.7)^a	0^C (8.34)^c	0
D56	100^A (76.4)^a	100^A (75.5)^a	100^A (89.3)^b	100^A (73.4)^a	0^B (14.1)^c	0
D70	100^A (92.6)^a	100^A (92.7)^a	100^A (93.5)^a	100^A (93.6)^a	60^B (63.6)^b	0
D84	100 (96.4)^a	100 (96.3)^a	100 (96.7)^a	100 (96.4)^a	100 (84.9)^b	0

Legend: Serology was performed using DAKO^® M.hyo ELISA (Oxoid Limited, Hampshire, UK) for Mycoplasma hyopneumoniae in each group (Groups A-E: n = 14 pigs per group; group F: n = 8 pigs)
Group A (F7.2C), B (F20.1L), C (B2V1W20 1A-F), D (J strain), E (positive control; non-vaccinated, challenge infected), F (negative control; non-vaccinated, non-challenged).
Sera with IP value > 50% was considered positive, IP values < 50% were considered negative. Intermediate IP values were considered doubtful and classified as negative.
Within a row, different superscript letters correspond to significant differences between the groups (P < 0.05): capital letters for the proportion of seropositive pigs, lowercase letters for the IP values. Group F was not included in the statistical analysis.

ISSN: 1746-6148