Infection with B. dendrobatidis ( Bd ). (a) Proportion of water frogs infected with Bd determined by quantitative rtPCR. (b) Infection intensities indicating Bd zoospore equivalents measured from swabs of Pelophylax esculentus and P. lessonae on day 40 post-exposure (feet swabbed) or on day 64 post-exposure (body swabbed after rinse). Frogs that were naturally infected with Bd (triangles) or uninfected before beginning the experiment (circles) are indicated. One P. esculentus died on day 80. This frog was naturally infected, and in addition to the highest Bd infection intensity, clinical signs indicative of chytridiomycosis included progressive shedding of skin in water, splayed limbs, inappetence, and lethargy.