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Table 1 Determination of the melting temperature obtained on different samples (porcine sapovirus- or norovirus-positive swine samples, human sapovirus- or norovirus-positive human samples)

From: Development and application of a SYBR green RT-PCR for first line screening and quantification of porcine sapovirus infection

Sample identifier

Infecting virus

GG

Intra-assay

Inter-assay

   

Assay 1

Assay 2

Assay 3

   
   

mean

SD

CV (%)

mean

SD

CV (%)

mean

SD

CV (%)

mean

SD

CV (%)

PC34

PoSaV

III

87

0

0

87.5

0

0

87.33

0.29

0.33

87.28

0.26

0.30

PC29

 

VI

86.5

0

0

87

0

0

87

0

0

86.83

0.25

0.29

PC42

 

VII

85

0

0

85.5

0

0

85.67

0.29

0.34

85.39

0.33

0.39

PC23

PoNoV

II

77.5

0

0

78

0

0

78.17

0.29

0.37

77.89

0.33

0.43

PC26

 

II

79

1.73

2.19

81.5

0

0

78

0

0

79.5

1.79

2.25

Be1

HuSaV

I

84.5

0

0

85

0

0

84.83

0.29

0.34

84.78

0.26

0.31

Be2

 

I

84

0

0

84.5

0

0

84.5

0

0

84.33

0.25

0.30

ISP475

HuNoV

II

82

0

0

82.5

0

0

82.5

0

0

82.33

0.25

0.30

CrH2

 

II

74.33

0.76

1.03

75

0

0

74.67

0.29

0.39

74.67

0.5

0.67

  1. Genogrouping is based on phylogenetic relationships in the polymerase sequence. GG: genogroup; PoSaV: porcine sapovirus; PoNoV: porcine norovirus; HuSaV: human sapovirus; HuNoV: human norovirus; SD: standard deviation; CV: coefficient of variation (%).
  2. Intra- and inter-assay variability recorded for the SYBR green real-time RT-PCR.