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Figure 4 | BMC Veterinary Research

Figure 4

From: Development and application of a SYBR green RT-PCR for first line screening and quantification of porcine sapovirus infection

Figure 4

A. Neighbor-joining phylogenetic tree based on partial RNA dependent RNA polymerase coding region (230 nt) of porcine norovirus strains identified in this study (in bold face), human and porcine norovirus reference strains, and other reference strains from the Caliciviridae family. Scale bar represents the phylogenetic distances expressed as units of expected nucleotide substitutions per site. Bootstrap values (1,000 replicates) are reported. Accession numbers in GenBank: Sw/NoV/OH-QW170/US [AY823306], Sw/NoV/MI-QW48/US [AY823303], Sw/NoV/OH-QW101/US [AY823304], Sw/NoV/Sw918/JP [AB074893], Sw/SaV/PEC Cowden strain/US [AF182760], Hu/NoV/Norwalk/US [M87661], Hu/NoV/Hawaii/US [U07611], Hu/NoV/Bristol/UK [X76716], LAGOVIRUS/RHDV-FRG strain [M67473], VESIVIRUS/FCV Urbana strain [L40021], Sw/NoV/sw50/NL [JN644277], Sw/NoV/sw68/NL [JN644278], Sw/NoV/sw76/NL [JN644279], Sw/NoV/sw86/NL [JN644280], Sw/NoV/sw90/NL [JN644281]. B. Multiple alignment performed with ClustalW on different reference porcine and human strains from the Sapovirus and Norovirus genera. Results were compared with those of the p289-290 primer pair.

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BMC Veterinary Research

ISSN: 1746-6148

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