Figure 3From: Development and application of a SYBR green RT-PCR for first line screening and quantification of porcine sapovirus infectionDetection limit of the SYBR Green real-time RT-PCR (A, C) in comparison with those of conventional RT-PCR (B, D). The two assays were performed on ten-fold dilutions of PC29 (from 100 to 10-6 in RNAse free water or from 100 to 10-4 in faecal suspension from a PoSaV-negative sample).Back to article page