Figure 3
Protective efficacy measured by post-mortem results, and cross-sectional comparison of IFN-γ results with vaccination outcome. (A) Correlation between pathological and bacteriological parameters of all goats (n = 20). Dashed line (y = −x + 4.16) indicates the threshold defined to divide vaccine responders (VR) and vaccine non-responders (VNR). Symbols represent vaccine cross-protection outcome: (■) unvaccinated controls (UC), (○) VR goats, (●) VNR goats. (B) Specific IFN-γ responses at week 28 of the experiment as determined by ELISA after stimulation of whole blood with PPD-B, ESAT-6/CFP-10, or Rv3615c. Results are represented in relation to vaccine cross-protective outcome and are expressed as mean ΔOD450 ± 95% confidence interval. Significances determined by non-parametric Kruskal-Wallis test: *p < 0.05.