Growth hormone secretagogue receptor expression in canine mammary carcinoma cell lines. (A) Representative pictures of canine mammary adenocarcinoma cell lines (CMT-W1, CMT-W2) and their lung metastases (CMT-W1M and CMT-W2M). Cancer cells showed a cytoplasmic staining reaction (brown color) of growth hormone secretagogue receptor (anti-Growth Hormone Secretagogue Receptor antibody obtained from Santa Cruz Biotechnology, USA was used). Pictures were obtained with Olympus BX60 microscope (at the magnification of x10). (B) The graph of integrated optical density (IOD) of growth hormone secretagogue receptor (GHS-R)-positive cells in examined canine cancer cell lines. The colorimetric intensity of the IHC-stained antygen spots was evaluated by a computer-assisted image analyzer (Olympus Microimage™ Image Analysis, software version 5.0 for windows, USA). Twenty pictures in each slide were analyzed. The results are presented as a mean (±SEM) from tree separate experiments. The statistical analysis was performed using Prism version 5.00 software (GraphPad Software, USA). The one-way ANOVA and unpaired t-test were applied. p < 0.05 was regarded as significant and marked as *.