Ghrelin promotes invasion of canine mammary carcinoma cells. (A) Quantification of invasion of examined cell lines (CMT-W1, CMT-W1M, CMT-W2, CMT-W2M) represented as relative florescent units obtained by florescent plate reader Infinite 200 PRO Tecan™ (Ex. 485, Em.530). Ghrelin treatment for 24 hrs at the concentration of 1 nM in CMT-W2 cell line and of 10 nM in remaining cell lines increased cancer cells invasion through membrane of Boyden chamber system pre-coated by BD Matrigel™ Matrix. The experiment has been conducted three times. The statistical analysis was performed using Prism version 5.00 software (GraphPad Software, USA). One-way ANOVA followed by Tukey HSD post-hoc test and unpaired t-test were applied. p < 0.05 was marked as *, p < 0.01 was marked as **. (B) Fluorescence microscopic analysis of the invaded through Matrigel layer CMTW-2 cancer cells using Olympus microscopy BX60 at x4 magnification.