Sensitivity and reproducibility of each real-time PCR method using a pBLV-IF. The copy number of pBLV-IF was determined by calculation and TaKaRa Cycleave PCR. One hundred copy of pBLV-IF was diluted 2-fold to construct the standard curve. Threshold values (Ct) were plotted against corresponding pBLV-IF copy numbers and the correlation coefficient (R2) was determined. The experiments were run in duplicate and independently repeated three times with the same dilutions. pBLV-IF standard curves were generated by using the results of CoCoMo-qPCR (A), the TaqMan MGB assay developed by Lew et al. (B), and TaKaRa Cycleave PCR (C).