Table 1 Characteristics of the two multiplex MLVA assays
Locus | Primers | MLVA primer sequence and label (5′-3′) |   Repeat sequence | Repeat length (bp) |   Sequencing primers (5′-3′) |
|---|---|---|---|---|---|
| Â | Multiplex assay 1 | Â | Â | Â | Â |
Ms27 (Cox2) | Forward primer | NED-CGCTATTTTTTCAGTTTTGAGTAA | TGAAGA | 6 | CAGTGCGTCCAGATTTCATTG |
| Â | Reverse primer | GTCGCAAACGTCGCACT | GTCCGGATACAGCTTGAAAAGT | ||
Ms28 (Cox5) | Forward primer | VIC-CGATGACGACAAAAAAGACT | TAAGAA | 6 | GCAGAAAAAGAACATGAATGTGATTGTG |
| Â | Reverse primer | GCGGTAATTACTGTAAATAAATACAAAGACA | GCCGGTAMCCTTCTCTAAATATTGCAA | ||
Ms34 (Cox1) | Forward primer | 6FAM-ATCAGCGACTCGAAGAAAAA | GAAAAG | 6 | CCAGTATCTCGTACGTCTCRATTT |
| Â | Reverse primer | AGGGTGACTTTTTCACTTAAAG | CGTTTGAACACGCAACTGTTTT | ||
| Â | Multiplex assay 2 | Â | Â | Â | Â |
Ms20b | Forward primer | VIC-TGTAACAGCACCGCCTGA | GGAAGAAGCGCCACCCG | 33 | ACAGGTGAGTCGCCATTAACG |
| Â | Reverse primer | GCTTTGCCCTTTCCTTGATTTTC | AAATAGGGTTGCCTCC | CCATTGGGATCAAGTTCATGACTAT | |
Ms24 (Cox4) | Forward primer | NED-CCATTGTGTAATTGACATGAAGAA | GACGGAA | 7 | TATGCGCATCTTCTCGGAGCA |
| Â | Reverse primer | GCCACACAACTCTGTTTTCAG | GCGCTCCTTCCTCCTGTAAG | ||
Ms31 (Cox7) | Forward primer | 6FAM-CCGGTATTCTAACCAACTGAAC | CAGAGGA | 7 | AGATAAAAAGAAAAAGCAACCCGTGAA |
| Â | Reverse primer | GAATCCCTCAGCACCCATTC | GGGTGCGTTTCCAAAAATAGTATAGG |