Figure 5

Macrophage-specific antigens expression in cancer cells in the co-culture conditions. Representative pictures obtained using confocal microscopy showing CD64 (A) and CD14 (B) expression (green fluorescence) in CMT-W2 canine mammary cancer cell line (stained using CMTMR) grown as a mono-culture (left panel) and as a co-culture with macrophages (right panel) for 72 hrs. Macrophages are marked by arrows. Cell imaging was performed by confocal laser scanning microscope FV-500 system (Olympus Optical Co, Germany). The cells were examined using the Fluoview program (Olympus Optical Co., Germany).