Figure 3

Monocytes differentiation toward macrophages after 72 hrs of culture. The pictures of CD64-positive monocytes sorted from mononuclear blood cells and cultured for 72 hrs showing their partial differentiation toward macrophages. (A) Macrophages grown as a mono-culture were stained using anti-CD64 FITC-conjugated antibodies (Becton Dickinson, USA). (B) Macrophages grown as a mono-culture were stained using anti-CD14 FITC-conjugated antibodies (LifeSpan Biosciences, USA). Cells were pictured by confocal laser scanning microscope FV-500 system (Olympus Optical Co, Germany). The cells were examined using the Fluoview program (Olympus Optical Co., Germany).