The expression of CSF1R and CD14 in canine mammary cancer tissues. Pictures obtained using Olympus BX60 microscope (x200) showing CSF1R (A) and CD14 (B) expression in canine mammary cancer tissues. These macrophage-specific antigens were detected not only on the monocytes/macrophages, but also on the cancer tissue (reflected as brown colour). Black arrows indicate macrophages, whereas white arrows indicate brown-coloured cancer cells. Tissue sections were stained using rabbit polyclonal CSF1R antibody and rabbit polyclonal CD14 antibody (both obtained from Abcam, United Kingdom). For the staining the EnVision kit (Dako, Denmark) was used (Labelled Polymers consist of secondary anti-rabbit antibodies conjugated with the Horseradish peroxidase HRP enzyme complex). To develop the coloured product, the 3,3'-Diaminobenzidine (DAB) substrate was used. Finally, the haematoxylin was used for nuclei counterstaining. The tissue details, procedure, and results have been described previously .