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Table 1 Intracellular localization of VP2 and various mutants of the NLS motif

From: Identification of the NLS and NES motifs of VP2 from chicken anemia virus and the interaction of VP2 with mini-chromosome maintenance protein 3

Name of mutants

Localizationa

NLS motifs of amino acids sequenceb, c

VP2-GFP

N

K 133 R 134 A 135 K136R137K138 L 139 D 140 Y 141 H 142 Y 143 S 144 Q 145 P 146 T 147 P 148 N 149 R 150 K 151 K 152

VP2 150-152A

N

K 133 R 134 A 135 K136R137K138 L 139 D 140 Y 141 H 142 Y 143 S 144 Q 145 P 146 T 147 P 148 N 149 A 150 A 151 A 152

VP2 136-138A

N

K 133 R 134 A 135 A136A137A138 L 139 D 140 Y 141 H 142 Y 143 S 144 Q 145 P 146 T 147 P 148 N 149 R 150 K 151 K 152

VP2 136-138A/150-152A

N

K 133 R 134 A 135 A136A137A138 L 139 D 140 Y 141 H 142 Y 143 S 144 Q 145 P 146 T 147 P 148 N 149 A 150 A 151 A 152

VP2 136-138A/133A

C

A 133 R 134 A 135 A 136 A 137 A 138

VP2 136-138A/134A

C

K 133 A 134 A 135 A 136 A 137 A 138

VP2 136-138A/133A/134A

C

A 133 A 134 A 135 A 136 A 137 A 138

VP2 133A

N

A 133 R 134 A 135 K 136 R 137 K 138

VP2 134A

N

K 133 A 134 A 135 K 136 R 137 K 138

VP2 133A/134A

N

A 133 A 134 A 135 K 136 R 137 K 138

  1. a. The N and C indicate nuclear and cytoplasm, respectively.
  2. b. The BiNLS1 and NLS2 motifs are indicated by underlining and bold, respectively.
  3. c. The amino acids of BiNLS1 are shown from K136 to K152 and of NLS2 are shown from K133 to K138.
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BMC Veterinary Research

ISSN: 1746-6148

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